Molecular cloning and analysis of the gene encoding the thermostable penicillin G acylase from Alcaligenes faecalis
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چکیده
منابع مشابه
Molecular cloning and analysis of the gene encoding the thermostable penicillin G acylase from Alcaligenes faecalis.
Alcaligenes faecalis penicillin G acylase is more stable than the Escherichia coli enzyme. The activity of the A. faecalis enzyme was not affected by incubation at 50 degrees C for 20 min, whereas more than 50% of the E. coli enzyme was irreversibly inactivated by the same treatment. To study the molecular basis of this higher stability, the A. faecalis enzyme was isolated and its gene was clon...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1997
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.63.9.3412-3418.1997